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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
inspiralis/Plasmid Substrates/600 µg/L0600
产品编号:L0600
市  场 价:¥7120.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$356.00
品      牌: inspiralis
公      司:inspiralis
公司分类:
inspiralis/Plasmid Substrates/600 µg/L0600
商品介绍

Plasmid Substrates

All our DNA substrates are available separately and have been prepared to a high standard for accurate quantification of results. They are guaranteed for use with all our topoisomerase assay kits, buffers and enzymes.

Click on the product headings below to view details and order online.

Supercoiled pBR322 DNA

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Supercoiled plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2.

Negatively-supercoiled pBR322 DNA is further treated so that the DNA is more supercoiled and has a narrow range of linking numbers making it an ideal substrate for relaxation reactions.

Positively-supercoiled plasmid pBR322 DNA is produced by treating relaxed pBR322 with reverse gyrase before final purification.

It is shipped on dry ice at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC

 

Technical Documents

Cat No.ProductPriceQuantity
S5001Supercoiled pBR322 plasmid50 µg£118
S2502Supercoiled pBR322 plasmid250 µg£430
S5003Supercoiled pBR322 plasmid500 µg£830
S1004Supercoiled pBR322 plasmid1 mg£1,595
POS5001Positively Supercoiled pBR322 plasmid50 µg£250
POS2502Positively Supercoiled pBR322 plasmid250 µg£990
Custom Quote

Relaxed pBR322 DNA

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Relaxed plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2 and the resulting supercoiled plasmid is relaxed using topoisomerase I. This is further purified and optimised for topoisomerase assays

It is shipped on dry ice at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC

0.5 µg of relaxed pBR322 when incubated with 1 U of DNA gyrase in a reaction volume of 30 µl at 37oC for 30 minutes in incubation buffer is completely converted to the supercoiled form.

Technical Documents

Cat No.ProductPriceQuantity
R5001Relaxed pBR322 plasmid50 µg£118
R2502Relaxed pBR322 plasmid250 µg£430
R5003Relaxed pBR322 plasmid500µg£830
R1004Relaxed pBR322 plasmid1 mg£1,595
Custom Quote

Linear pBR322 DNA

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Linear plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2.

It is linearised by incubation with EcoRI and further concentrated and purified to a final concentration of of 1 mg/ml in TE (10 mM Tris-HCl (pH 7.5), 1 mM EDTA). Store at 4oC

It is shipped on dry ice. 

Technical Documents

Cat No.ProductPriceQuantity
L0150Linear pBR322150 µg£100
L0300Linear pBR322300 µg£178
L0600Linear pBR322600 µg£356
L1000Linear pBR3221 mg µg£556
Custom Quote

Linking Number Plasmid

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Plasmid pBR322 is partially negatively supercoiled to give specific linking numbers so that each marker has a range of 5-6 linking numbers. The kit contains a set of markers from relaxed plasmid covering approximately 23 linking numbers. Please enquire about single markers.

Technical Documents

Cat No.ProductPriceQuantity
LNM001Linking Number markersSet of 10 (20 µl of each at 1 µg/µl) enough for 10 gels£240
Custom Quote

References

  1. Sambrook, J., Fritsch, E.F. & Maniatis, T (1989). Molecular cloning: a laboratory manual,2nd ed., Cold Spring Harbor Press. Cold Spring Harbor, NY.
  2. Boros, I., Pósfai, G. & Venetianer, P. (1984). High-copy number derivatives of the plasmid cloning vector pBR322. Gene 30, 257-260
  3. Trask, D. K. & Muller, M. T. (1983) Biochemical characterization of topoisomerase I purified from avian erythrocytes. Nucleic Acids Res. 11, 2779-2800
  4. Shapiro, T.A., Klein, V.A. and Englund, P.T. (1999) Isolation of kinetoplast DNA, in DNA Topoisomerase Protocols Vol.I (Bjornsti, M-A and Osheroff, N., eds.). Humana Press Inc., N. Jersey, pp. 61-68
品牌介绍

Inspiralis


Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。


We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.


Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.


产品列表:



No.

品牌

货号

名称

规格

1

Inspiralis

7F001

7F11 Monoclonal

0.1ml

2

Inspiralis

PA001

Anti-Gyr A Polyclonal

0.1 ml

3

Inspiralis

HT001

Anti-human topo II polyclonal - 0.1mL

0.1 ml

4

Inspiralis

G1001

E.coli gyrase

100 U

5

Inspiralis

T4001

E. coli Topo IV

100 U

6

Inspiralis

SAG4001

S. aureus gyrase

100 U

7

Inspiralis

SAT4001

S. aureus Topo IV

100 U

8

Inspiralis

MTG001

M. tuberculosis Gyrase (HIS)

100 U

9

Inspiralis

MTT01

M.tuberculosis Topo I

100 U

10

Inspiralis

PAG1001

P. aeruginosa Gyrase

100 U

11

Inspiralis

PAT4001

P. aeruginosa Topo IV

100 U

12

Inspiralis

SPG1001

S. pneumoniae Gyrase

100 U

13

Inspiralis

SPT4001

S. pneumoniae Topo IV

100 U

14

Inspiralis

ABG1001

A. baumannii Gyrase

100 U

15

Inspiralis

ABT4001

A. baumannii Topo IV

100 U



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