M. tuberculosis Topo I
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M.tuberculosis Topo I
Mycobacterium tuberculosis topoisomerase I is a bacterial type I topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by chromatography.
It is supplied in Dilution Buffer. Store at -80 °C.
The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately. 1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37 °C.
See technical documents below for more detailed information and lot specific activities.
Technical Documents
M.tuberculosis Topo I Relaxation Kit 1
These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
Technical Documents
High/Medium-Throughput Assay Kit - M.tuberculosis Topo I
The kit is supplied with sufficient M.tuberculosis topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a minimum concentration of 5 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use. The streptavidin-coated plate is also included.
More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".
Kit issued with limited licence for individual use only.
Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)
Technical Documents
Inspiralis
Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。
We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.
Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.
产品列表：
No. | 品牌 | 货号 | 名称 | 规格 |
1 | Inspiralis | 7F001 | 7F11 Monoclonal | 0.1ml |
2 | Inspiralis | PA001 | Anti-Gyr A Polyclonal | 0.1 ml |
3 | Inspiralis | HT001 | Anti-human topo II polyclonal - 0.1mL | 0.1 ml |
4 | Inspiralis | G1001 | E.coli gyrase | 100 U |
5 | Inspiralis | T4001 | E. coli Topo IV | 100 U |
6 | Inspiralis | SAG4001 | S. aureus gyrase | 100 U |
7 | Inspiralis | SAT4001 | S. aureus Topo IV | 100 U |
8 | Inspiralis | MTG001 | M. tuberculosis Gyrase (HIS) | 100 U |
9 | Inspiralis | MTT01 | M.tuberculosis Topo I | 100 U |
10 | Inspiralis | PAG1001 | P. aeruginosa Gyrase | 100 U |
11 | Inspiralis | PAT4001 | P. aeruginosa Topo IV | 100 U |
12 | Inspiralis | SPG1001 | S. pneumoniae Gyrase | 100 U |
13 | Inspiralis | SPT4001 | S. pneumoniae Topo IV | 100 U |
14 | Inspiralis | ABG1001 | A. baumannii Gyrase | 100 U |
15 | Inspiralis | ABT4001 | A. baumannii Topo IV | 100 U |
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