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inspiralis/M. tuberculosis Topo I/500 U of M.tuberculosis Topo I and 250 µg of Supercoiled DNA/MTTR02

inspiralis/M. tuberculosis Topo I/500 U of M.tuberculosis Topo I and 250 µg of Supercoiled DNA/MTTR02
  • inspiralis/M. tuberculosis Topo I/500 U of M.tuberculosis Topo I and 250 µg of Supercoiled DNA/MTTR02
商品介绍

M. tuberculosis Topo I

Click on the product headings below to view details and order online.

M.tuberculosis Topo I

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Mycobacterium tuberculosis topoisomerase I is a bacterial type I topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by chromatography.

It is supplied in Dilution Buffer. Store at -80 °C.

The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately. 1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
MTT01M.tuberculosis Topo I100U£110
MTT02M.tuberculosis Topo I500U£405
Custom Quote

M.tuberculosis Topo I Relaxation Kit 1

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These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.

Technical Documents

Cat No.ProductPriceQuantity
MTTR01M.tuberculosis Relaxation Kit 1100 U of M.tuberculosis Topo I and 50 µg of Supercoiled DNA£193
MTTR02M.tuberculosis Relaxation Kit 2500 U of M.tuberculosis Topo I and 250 µg of Supercoiled DNA£703
Custom Quote

High/Medium-Throughput Assay Kit - M.tuberculosis Topo I

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The kit is supplied with sufficient M.tuberculosis topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a minimum concentration of 5 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use. The streptavidin-coated plate is also included.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
MTTH01HTS Assay Kit - M.tuberculosis Topo I1-5 KitsMaterial for 100 assays per plate£410
MTTH02HTS Assay Kit - M.tuberculosis Topo I6+ KitsMaterial for 100 assays.per plate£341
Custom Quote
品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



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inspiralis抗人类topo II抗体

所有抗体均储存在pH 7.2的磷酸盐缓冲盐水(PBS)中。储存在-20℃。(至少在未经稀释的情况下稳定12个月)。

单击下面的产品标题以查看详细信息并在线订购。

抗人topo II多克隆抗体

针对完整的人拓扑异构酶II蛋白,从兔血中分离出抗人topo II多克隆抗体。抗体的浓度需要约1:1,000的稀释才能用于蛋白质印迹。


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