S. pneumoniae Topoisomerase IV

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S. pneumoniae Topoisomerase IV

Topo IV (from Streptococcus pneumoniae) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.	Product	Price	Quantity
SPT4001	S. pneumoniae Topo IV100U	£136
SPT4002	S. pneumoniae Topo IV500U	£498

S. pneumoniae Topoisomerase IV Relaxation Assay Kits

These contain S. pneumoniae topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.

Technical Documents

Cat No.	Product	Price	Quantity
SPR4001	S.pneumoniae Topo IV RelaxationAssay Kit 1100 U of S.pneumoniae Topo IV and 50 µg of Supercoiled DNA	£220
SPR4002	S.pneumoniae Topo IV RelaxationAssayKit 2500 U of S. pneumoniae Topo IV and 250 µg of Supercoiled DNA	£817

Custom Quote

S. pneumoniae Topoisomerase IV Decatenation Assay Kits

These contain S. pneumoniae topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.

Technical Documents

Cat No.	Product	Price	Quantity
SPD4001	S.pneumoniae Topo IV Decatenation AssayKit 1100 U of S.pneumoniae Topo IV and 20 µg of kDNA	£220
SPD4002	S.pneumoniae Topo IV Decatenation Assay Kit 2500 U of S.pneumoniae Topo IV and 100 µg of kDNA	£817

Custom Quote

品牌介绍

Inspiralis

Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。

We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.

Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.

产品列表：

No.	品牌	货号	名称	规格
1	Inspiralis	7F001	7F11 Monoclonal	0.1ml
2	Inspiralis	PA001	Anti-Gyr A Polyclonal	0.1 ml
3	Inspiralis	HT001	Anti-human topo II polyclonal - 0.1mL	0.1 ml
4	Inspiralis	G1001	E.coli gyrase	100 U
5	Inspiralis	T4001	E. coli Topo IV	100 U
6	Inspiralis	SAG4001	S. aureus gyrase	100 U
7	Inspiralis	SAT4001	S. aureus Topo IV	100 U
8	Inspiralis	MTG001	M. tuberculosis Gyrase (HIS)	100 U
9	Inspiralis	MTT01	M.tuberculosis Topo I	100 U
10	Inspiralis	PAG1001	P. aeruginosa Gyrase	100 U
11	Inspiralis	PAT4001	P. aeruginosa Topo IV	100 U
12	Inspiralis	SPG1001	S. pneumoniae Gyrase	100 U
13	Inspiralis	SPT4001	S. pneumoniae Topo IV	100 U
14	Inspiralis	ABG1001	A. baumannii Gyrase	100 U
15	Inspiralis	ABT4001	A. baumannii Topo IV	100 U