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℡ 4000-520-616
℡ 4000-520-616
inspiralis/E. coli Topoisomerase IV/For 500 assays/TCE002
产品编号:TCE002
市  场 价:¥8040.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$402.00
品      牌: inspiralis
公      司:inspiralis
公司分类:
inspiralis/E. coli Topoisomerase IV/For 500 assays/TCE002
商品介绍

E. coli Topoisomerase IV

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E. coli Topoisomerase IV

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Topo IV (from Escherichia coli) is prepared by overexpressing the parC and parE subunits in E. coli and purifying them by methods adapted from Peng and Marians, 19991.

The parC and parE subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
T4001E. coli Topo IV100 U£133
T4002E. coli Topo IV500 U£499
T4003E. coli Topo IV1000 U£995
T4004E. coli Topo IV2000 U£1,875
Custom Quote

E. coli Topoisomerase IV Relaxation Assay Kits

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These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 

Technical Documents

Cat No.ProductPriceQuantity
R4001E. coli Topo IV Relaxation Kit 1100 U of E. coli Topo IV and 50 µg of Supercoiled DNA£220
R4002E. coli Topo IV Relaxation Kit 2500 U of E. coli Topo IV and 50 µg of Supercoiled DNA£810
R4003E. coli Topo IV Relaxation Kit 31000 U of E. coli Topo IV and 50 µg of Supercoiled DNA£1,603
R4004E. coli Topo IV Relaxation Kit 42000 U of E. coli Topo IV and 50 µg of Supercoiled DNA£3,016
Custom Quote

E. coli Topoisomerase IV DecatenationAssay Kits

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These contain the enzyme and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. 

Technical Documents

Cat No.ProductPriceQuantity
D4001E. coli Topo IV Decatenation Kit 1100 U of E. coli Topo IV and 20 µg kDNA£220
D4002E. coli Topo IV Decatenation Kit 2500 U of E. coli Topo IV and 100 µg kDNA£810
D4003E. coli Topo IV Decatenation Kit 31000 U of E. coli Topo IV and 200 µg kDNA£1,603
D4004E. coli Topo IV Decatenation Kit 42000 U of E. coli Topo IV and 400 µg kDNA£3,016
Custom Quote

E. coli Topoisomerase IV Assay Kits for Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.

Technical Documents

Cat No.ProductPriceQuantity
TCE001E. coli Topo IV Assay Kit 1 for Cell ExtractsFor 100 assays£136
TCE002E. coli Topo IV Assay Kit 2 for Cell ExtractsFor 500 assays£402
TCE003E. coli Topo IV Assay Kit 3 for Cell ExtractsFor 1000 assays£799
TCE004E. coli Topo IV Assay Kit 4 for Cell ExtractsFor 2000 assays£1,527
Custom Quote

E. coli Topoisomerase IV Cleavage Assay Kits

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These kits are designed specifically for cleavage reactions. They contain topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to the enzyme and linearised pBR322 marker.

Cleavage specific enzyme available separately on request.

Technical Documents

Cat No.ProductPriceQuantity
TCK001E. coli Topo IV Cleavage Kit 1100 U of E. coli Topo IV and 50 µg of supercoiled DNA£399
Custom Quote

E.coli Topoisomerase IV ATPase kits

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These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of topoisomerase IV by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling.

These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.

Technical Documents

Cat No.ProductPriceQuantity
ATPECT001Kit for 100 ATPase assaysFor determination of DNA-dependent ATPase activity; contains E. coli topoIV, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.£1,472
Custom Quote

High / Medium-Throughput Assay Kit - E. coli Topoisomerase IV

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The kit is supplied with sufficient E. coli topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
TRIV01HTS Assay Kit - E. coli Topo IV 1-5 KitsFor 100 assays per plate£440
TRIV02HTS Assay Kit - E. coli Topo IV 6+ KitsFor 100 assays per plate£346
Custom Quote

References

  1. Peng, H. and Marians, K.J. (1999) Overexpression and purification of bacterial topoisomerase IV, in DNA Topoisomerase Protocols Vol. I (Bjornsti, M-A., and Osheroff, N. eds.), Humana Press, Totowa, N.Jersey pp.163-169
  2. Maxwell, A., Burton, N.P. and O"Hagan, N. (2006) High-throughput assays for DNA gyrase and other topoisomerases. Nucleic Acid Res. 34 (15), e104
品牌介绍

Inspiralis


Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。


We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.


Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.


产品列表:



No.

品牌

货号

名称

规格

1

Inspiralis

7F001

7F11 Monoclonal

0.1ml

2

Inspiralis

PA001

Anti-Gyr A Polyclonal

0.1 ml

3

Inspiralis

HT001

Anti-human topo II polyclonal - 0.1mL

0.1 ml

4

Inspiralis

G1001

E.coli gyrase

100 U

5

Inspiralis

T4001

E. coli Topo IV

100 U

6

Inspiralis

SAG4001

S. aureus gyrase

100 U

7

Inspiralis

SAT4001

S. aureus Topo IV

100 U

8

Inspiralis

MTG001

M. tuberculosis Gyrase (HIS)

100 U

9

Inspiralis

MTT01

M.tuberculosis Topo I

100 U

10

Inspiralis

PAG1001

P. aeruginosa Gyrase

100 U

11

Inspiralis

PAT4001

P. aeruginosa Topo IV

100 U

12

Inspiralis

SPG1001

S. pneumoniae Gyrase

100 U

13

Inspiralis

SPT4001

S. pneumoniae Topo IV

100 U

14

Inspiralis

ABG1001

A. baumannii Gyrase

100 U

15

Inspiralis

ABT4001

A. baumannii Topo IV

100 U



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