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inspiralis/Plasmid Substrates/1 mg/S1004

  • 产品编号: S1004
  • 美  元  价: $1595.00
  • 市  场  价: ¥31900.00
  • 品       牌: inspiralis
  • 产       地: 美国(厂家直采)
  • 公       司: inspiralis
  • 产品分类:
  • 公司分类: DNA Substrates and Markers
inspiralis/Plasmid Substrates/1 mg/S1004
  • inspiralis/Plasmid Substrates/1 mg/S1004
商品介绍

Plasmid Substrates

All our DNA substrates are available separately and have been prepared to a high standard for accurate quantification of results. They are guaranteed for use with all our topoisomerase assay kits, buffers and enzymes.

Click on the product headings below to view details and order online.

Supercoiled pBR322 DNA

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Supercoiled plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2.

Negatively-supercoiled pBR322 DNA is further treated so that the DNA is more supercoiled and has a narrow range of linking numbers making it an ideal substrate for relaxation reactions.

Positively-supercoiled plasmid pBR322 DNA is produced by treating relaxed pBR322 with reverse gyrase before final purification.

It is shipped on dry ice at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC

 

Technical Documents

Cat No.ProductPriceQuantity
S5001Supercoiled pBR322 plasmid50 µg£118
S2502Supercoiled pBR322 plasmid250 µg£430
S5003Supercoiled pBR322 plasmid500 µg£830
S1004Supercoiled pBR322 plasmid1 mg£1,595
POS5001Positively Supercoiled pBR322 plasmid50 µg£250
POS2502Positively Supercoiled pBR322 plasmid250 µg£990
Custom Quote

Relaxed pBR322 DNA

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Relaxed plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2 and the resulting supercoiled plasmid is relaxed using topoisomerase I. This is further purified and optimised for topoisomerase assays

It is shipped on dry ice at a concentration of 1mg/ml in TE (10mM Tris-HCl (pH 7.5), 1mM EDTA). Store at 4oC

0.5 µg of relaxed pBR322 when incubated with 1 U of DNA gyrase in a reaction volume of 30 µl at 37oC for 30 minutes in incubation buffer is completely converted to the supercoiled form.

Technical Documents

Cat No.ProductPriceQuantity
R5001Relaxed pBR322 plasmid50 µg£118
R2502Relaxed pBR322 plasmid250 µg£430
R5003Relaxed pBR322 plasmid500µg£830
R1004Relaxed pBR322 plasmid1 mg£1,595
Custom Quote

Linear pBR322 DNA

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Linear plasmid pBR322 DNA is produced by the large-scale alkaline-lysis method ( Sambrook et al., 1989)1 from a high-copy derivative of pBR322 (Boros et al., 1984)2.

It is linearised by incubation with EcoRI and further concentrated and purified to a final concentration of of 1 mg/ml in TE (10 mM Tris-HCl (pH 7.5), 1 mM EDTA). Store at 4oC

It is shipped on dry ice. 

Technical Documents

Cat No.ProductPriceQuantity
L0150Linear pBR322150 µg£100
L0300Linear pBR322300 µg£178
L0600Linear pBR322600 µg£356
L1000Linear pBR3221 mg µg£556
Custom Quote

Linking Number Plasmid

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Plasmid pBR322 is partially negatively supercoiled to give specific linking numbers so that each marker has a range of 5-6 linking numbers. The kit contains a set of markers from relaxed plasmid covering approximately 23 linking numbers. Please enquire about single markers.

Technical Documents

Cat No.ProductPriceQuantity
LNM001Linking Number markersSet of 10 (20 µl of each at 1 µg/µl) enough for 10 gels£240
Custom Quote

References

  1. Sambrook, J., Fritsch, E.F. & Maniatis, T (1989). Molecular cloning: a laboratory manual,2nd ed., Cold Spring Harbor Press. Cold Spring Harbor, NY.
  2. Boros, I., Pósfai, G. & Venetianer, P. (1984). High-copy number derivatives of the plasmid cloning vector pBR322. Gene 30, 257-260
  3. Trask, D. K. & Muller, M. T. (1983) Biochemical characterization of topoisomerase I purified from avian erythrocytes. Nucleic Acids Res. 11, 2779-2800
  4. Shapiro, T.A., Klein, V.A. and Englund, P.T. (1999) Isolation of kinetoplast DNA, in DNA Topoisomerase Protocols Vol.I (Bjornsti, M-A and Osheroff, N., eds.). Humana Press Inc., N. Jersey, pp. 61-68
品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

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inspiralis突变体的克隆与表达

尽管我们拥有来自许多生物体和一系列突变体的拓扑异构酶,但是如果您感兴趣的蛋白似乎尚不可用,那么我们应该能够为您制造它。如果您告诉我们您感兴趣的蛋白质,那么我们可以克隆这些亚基,进行表达和纯化,然后为您提供特色产品。

如果您对我们拥有的特定蛋白质突变体感兴趣,但似乎不在我们的清单中,请查询,我们可能会提供该蛋白质,如果没有,我们可以为您提供。 

我们还可以提供特殊用途的蛋白质,例如X射线晶体学。


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