T4 DNA Ligase

Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).

T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA.  It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.

T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)

Technical Documents

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Cat No.	Product	Price	Quantity
TDL1	Kit 180,000U T4 DNA ligase (2000U/ul), 10X buffer	£40
TDL2	Kit 2400,000U T4 DNA ligase (2000U/ul), 10X buffer	£130
TDL3C	Kit3400,000U T4 DNA ligase (13,000U/ul), 10X buffer	£130

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Inspiralis

Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。

We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.

Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.

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