Human Topoisomerase I
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Human Topoisomerase I
Human Topoisomerase I is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996. The enzyme is supplied μl in Dilution Buffer.
Store at -80°C. It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.
See technical documents below for more detailed information and lot specific activities.
Technical Documents
Human Topoisomerase I Relaxation Assay Kits
These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
Technical Documents
Human Topoisomerase I Assay Kits for Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing human topo I. They contain supercoiled DNA substrate, Assay buffer, Dilution buffer and relaxed DNA marker.
The kit components are based on the standard human topo I assay whch contains 500 ng substrate DNA per assay.
Technical Documents
High / Medium-Throughput Assay Kit - Human Topoisomerase I
The kit is supplied with sufficient human topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".
Kit issued with limited licence for individual use only.
Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230).
Technical Documents
Inspiralis
Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。
We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.
Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.
产品列表：
No. | 品牌 | 货号 | 名称 | 规格 |
1 | Inspiralis | 7F001 | 7F11 Monoclonal | 0.1ml |
2 | Inspiralis | PA001 | Anti-Gyr A Polyclonal | 0.1 ml |
3 | Inspiralis | HT001 | Anti-human topo II polyclonal - 0.1mL | 0.1 ml |
4 | Inspiralis | G1001 | E.coli gyrase | 100 U |
5 | Inspiralis | T4001 | E. coli Topo IV | 100 U |
6 | Inspiralis | SAG4001 | S. aureus gyrase | 100 U |
7 | Inspiralis | SAT4001 | S. aureus Topo IV | 100 U |
8 | Inspiralis | MTG001 | M. tuberculosis Gyrase (HIS) | 100 U |
9 | Inspiralis | MTT01 | M.tuberculosis Topo I | 100 U |
10 | Inspiralis | PAG1001 | P. aeruginosa Gyrase | 100 U |
11 | Inspiralis | PAT4001 | P. aeruginosa Topo IV | 100 U |
12 | Inspiralis | SPG1001 | S. pneumoniae Gyrase | 100 U |
13 | Inspiralis | SPT4001 | S. pneumoniae Topo IV | 100 U |
14 | Inspiralis | ABG1001 | A. baumannii Gyrase | 100 U |
15 | Inspiralis | ABT4001 | A. baumannii Topo IV | 100 U |
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