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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
inspiralis/Human Topoisomerase I/100 U of human topo I and 50 µg of supercoiled DNA/HTR101
产品编号:HTR101
市  场 价:¥3900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$195.00
品      牌: inspiralis
公      司:inspiralis
公司分类:
inspiralis/Human Topoisomerase I/100 U of human topo I and 50 µg of supercoiled DNA/HTR101
商品介绍

Human Topoisomerase I

Click on the product headings below to view details and order online.

Human Topoisomerase I

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Human Topoisomerase I is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996. The enzyme is supplied μl in Dilution Buffer.

Store at -80°C. It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
HT101Human Topo I100 U£75
HT105Human Topo I500 U£230
HT110Human Topo I1000 U£385
HT120Human Topo I2000 U£640
Custom Quote

Human Topoisomerase I Relaxation Assay Kits

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These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 

Technical Documents

Cat No.ProductPriceQuantity
HTR101Human Topo I Relaxation Assay Kit 1100 U of human topo I and 50 µg of supercoiled DNA£195
HTR102Human Topo I Relaxation AssayKit 2500 U of human topo I and 250 µg of supercoiled DNA£615
HTR103Human Topo I Relaxation AssayKit 31000 U of human topo I and 500 µg of supercoiled DNA£1,150
HTR104Human Topo I Relaxation AssayKit 42000 U of human topo I and 1 mg of supercoiled DNA£1,800
Custom Quote

Human Topoisomerase I Assay Kits for Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing human topo I. They contain supercoiled DNA substrate, Assay buffer, Dilution buffer and relaxed DNA marker.

The kit components are based on the standard human topo I assay whch contains 500 ng substrate DNA per assay.

Technical Documents

Cat No.ProductPriceQuantity
TRAK1Human Topo I Cell Extract Assay Kit 1Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 100 assays£162
TRAK2Human Topo I Cell Extract AssayKit 2Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 250 assays£409
Custom Quote

High / Medium-Throughput Assay Kit - Human Topoisomerase I

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The kit is supplied with sufficient human topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230).

Technical Documents

Cat No.ProductPriceQuantity
TRT101HTS Assay Kit - Human Topo I 1-5 kitsfor 100 assays per plate£430
TRT102HTS Assay Kit - Human Topo I 6+ Kitsfor 100 assays per plate£346
Custom Quote
品牌介绍

Inspiralis


Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。


We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.


Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.


产品列表:



No.

品牌

货号

名称

规格

1

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7F001

7F11 Monoclonal

0.1ml

2

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3

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0.1 ml

4

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5

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T4001

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100 U

6

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SAG4001

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100 U

7

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SAT4001

S. aureus Topo IV

100 U

8

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MTG001

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100 U

9

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MTT01

M.tuberculosis Topo I

100 U

10

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PAG1001

P. aeruginosa Gyrase

100 U

11

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PAT4001

P. aeruginosa Topo IV

100 U

12

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SPG1001

S. pneumoniae Gyrase

100 U

13

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SPT4001

S. pneumoniae Topo IV

100 U

14

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ABG1001

A. baumannii Gyrase

100 U

15

Inspiralis

ABT4001

A. baumannii Topo IV

100 U



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