Specific Gyrase Protein Domains

All our gyrase subunits are supplied at a concentration of 1 mg/ml unless otherwise specified and are purified to > 95% as assessed by SDS-PAGE. We have an extensive collection of mutants not listed here so please contact us for a full list. Different quantities and concentrations can be supplied on request.

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Gyrase Subunits

For studies involving mutant drug-resistant E. coli gyrase subunits the wild-type subunits are available separately in the following amounts. The subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. Other quantities and concentrations are available as are the individual subunits from S. aureus and M. tuberculosis gyrases; please enquire for further information.

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Cat No.	Product	Price	Quantity
SUBA001	E. coli Gyrase A0.1 mg	POA
SUBA005	E. coli Gyrase A0.5 mg	POA
SUBA100	E. coli Gyrase A1.0 mg	POA
SUBB001	E. coli Gyrase B0.1 mg	POA
SUBB005	E. coli Gyrase B0.5 mg	POA
SUBB100	E. coli Gyrase B1.0 mg	POA

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Purified Gyrase Domains

For structural studies the following E. coli gyrase domains are available: GyrB43, GyrB24 and GyrA59. Domains supplied at a concentration of 1 mg/ml purified to > 95% as assessed by SDS-PAGE. (see gel below for typical levels of purity)

Larger quantities/different concentrations are available, please enquire.

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Cat No.	Product	Price	Quantity
A59001	Gyrase A590.1 mg	POA
A59005	Gyrase A590.5 mg	POA
A59100	Gyrase A591.0 mg	POA
B43001	Gyrase B430.1 mg	POA
B43005	Gyrase B430.5 mg	POA
B43100	Gyrase B431.0 mg	POA
B24001	Gyrase B240.1 mg	POA
B24005	Gyrase B240.5 mg	POA
B24100	Gyrase B241.0 mg	POA

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Drug-Resistant Topoisomerases

We offer various quinolone- and coumarin-resistant topoisomerase mutants includingE.coli, S.aureus and other organisms. The proteins can be supplied as separate subunits or as reconstituted enzyme. Typically the quinolone-resistant mutants confer 50-100 X more resistance to fluoroquinolones than the wild-type. We have more than 25 mutants in different topoisomerases and can make other mutants as requested. 

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Select a document...S83L Quinolone Resistant E.coli gyrase Lot# 041017 Certificate of AnalysisView

Cat No.	Product	Price	Quantity
AS83W001	E.coli GyrASer83TrpPlease enquire	POA
AS83L001	E.coli GyrASer83LeuPlease enquire	POA
BD426N001	E.coli GyrBAsp426AsnPlease enquire	POA
SAAS84L01	S.aureus GyrASer84LeuPlease enquire	POA

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Inspiralis

Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。

We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.

Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.

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