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inspiralis/Human Topoisomerase II alpha and beta/For 100 assays per plate/TRT202

inspiralis/Human Topoisomerase II alpha and beta/For 100 assays per plate/TRT202
  • inspiralis/Human Topoisomerase II alpha and beta/For 100 assays per plate/TRT202
商品介绍

Human Topoisomerase II alpha and beta

Click on the product headings below to view details and order online.

Human Topoisomerase II Alpha

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Human topoisomerase II alpha is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied in Dilution Buffer.

Store at -80°C.

It is recommended that larger pack sizes (500U and above) of the enzyme are aliquoted to avoid repeated freeze-thaw cycles.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
HT201Human Topo II alpha100 U£75
HT205Human Topo II alpha500 U£230
HT210Human Topo II alpha1000 U£385
HT220Human Topo II alpha2000 U£640
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Human Topoisomerase II Beta

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Human topoisomerase II beta is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied at a concentration of 2-10 U/μl in Dilution Buffer.

Store at -80°C. (Stable for 3 months undiluted).

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.

Technical Documents

Cat No.ProductPriceQuantity
HTB201Human Topo II beta100U£75
HTB205Human Topo II beta500U£230
HTB210Human Topo II beta1000U£385
HTB220Human Topo II beta2000U£640
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Human Topoisomerase II Decatenation Assay Kits

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These contain human topo II and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of human topo II will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No.ProductPriceQuantity
HTK201Human Topo II alpha Decatenation Assay Kit 1Contains 100 U of human topo II alpha and 20 µg kDNA£195
HTK202Human Topo II alpha Decatenation Assay Kit 2Contains 500 U of human topo II alpha and 100 µg kDNA£615
HTK203Human Topo II alpha Decatenation Assay Kit 3Contains 1000 U of human topo II alpha and 200 µg kDNA£970
HTK204Human Topo II alpha Decatenation Assay Kit 4Contains 2000 U of human topo II alpha and 400 µg kDNA£1,540
HTKB201Human Topo II beta Decatenation Assay Kit 1Contains 100 U of human topo II beta and 20 µg kDNA£195
HTKB202Human Topo II beta Decatenation Assay Kit 2Contains 500 U of human topo II beta and 100 µg kDNA£615
HTKB203Human Topo II beta Decatenation Assay Kit 3Contains 1000 U of human topo II beta and 200 µg kDNA£970
HTKB204 Human Topo II beta Decatenation Assay Kit 4Contains 2000 U of human topo II beta and 400 µg kDNA£1,540
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Human Topoisomerase II Relaxation Assay Kits

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These contain human topo II and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo II will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.  The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No.ProductPriceQuantity
HTR201Human Topo II alpha Relaxation Assay Kit 1Contains 100 U of human topo II alpha and 50 µg supercoiled DNA£195
HTR202Human Topo II alpha Relaxation Assay Kit 2Contains 500 U of human topo II alpha and 250 µg supercoiled DNA£615
HTR203Human Topo II alpha Relaxation Assay Kit 3Contains 1000 U of human topo II alpha and 500 µg supercoiled DNA£1,150
HTR204 Human Topo II alpha Relaxation Assay Kit 4Contains 2000 U of human topo II alpha and 1 mg supercoiled DNA£1,800
HTRB201Human Topo II beta Relaxation Assay Kit 1Contains 100 U of human topo II beta and 50ug supercoiled DNA£195
HTRB202Human Topo II beta Relaxation Assay Kit 2Contains 500 U of human topo II beta and 250 µg supercoiled DNA£615
HTRB203Human Topo II beta Relaxation Assay Kit 3Contains 1000 U of human topo II beta and 500 µg supercoiled DNA£1,150
HTRB204Human Topo II beta Relaxation Assay Kit 4Contains 2000 U of human topo II beta and 1 mg supercoiled DNA£1,800
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Human Topoisomerase II Assay Kits For Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing human topo II. They contain kDNA substrate, Assay buffer, Dilution buffer, decatenated and linear DNA markers and stop buffer / loading dye.

The kit components are based on the standard assay which contains 500 ng substrate DNA per assay.

Technical Documents

Cat No.ProductPriceQuantity
HDAK1Kit 1Contains sufficient kDNA, assay and dilution buffers for 100 assays as well as linear and decatenated marker DNA£164
HDAK2Kit 2Contains linear and decatenated marker DNA£410
HDAK3Kit 3Contains sufficient kDNA, assay and dilution buffers for 500 assays as well as linear and decatenated marker DNA£525
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High / Medium-Throughput Assay Kit - Human Topoisomerase II

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The kit is supplied with sufficient human topo II enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.  The kits are available with either the alpha or beta forms of the enzyme. 

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
TRT201HTS Assay Kit - Human Topo II 1-5 KitsFor 100 assays per plate£430
TRT202HTS Assay Kit - Human Topo II 6+ KitsFor 100 assays per plate£346
TRTB201HTS Assay Kit - Human Topo II Beta 1-5 KitsFor 100 assays per plate£430
TRTB202HTS Assay Kit - Human Topo II Beta 6+ KitsFor 100 assays per plate£346
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Human Topoisomerase II alpha 453 subunit

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Human topoisomerase II alpha amino acids 1-453. This contains the ATPase domain, the activity of which can be stimulated by DNA (see Campbell and Maxwell (2002), J.Biol.Chem. 320 p.171.  The protein is expressed in E.coli.

Technical Documents

Cat No.ProductPriceQuantity
HT453Human Topo II 453 domainPlease enquirePOA
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品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

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inspiralis基于凝胶的分析

我们提供一系列基于凝胶的测定法,并具有多年与制药公司和学术界的客户合作的经验。

  • DNA旋转酶超螺旋

  • DNA拓扑异构酶I松弛

  • DNA拓扑异构酶II松弛

  • DNA拓扑异构酶II变性

  • DNA拓扑异构酶IV松弛

  • DNA拓扑异构酶IV脱落

这些都使用琼脂糖凝胶将反应产物与底物分离。在管中进行超螺旋和松弛反应,然后使用琼脂糖凝胶分离产物。分离使得可以看到不同的拓扑异构酶中间体以及超螺旋和松弛质粒。

蜕皮反应可测量从kris中的小环和大环复合物中DNA小环的解链,该复合体已从Crithidia fascicula中分离出来  与松弛反应相比,某些酶在脱脂中具有更高的活性。

与基于微量滴定板的测定相比,基于凝胶的测定的结果的视觉性质可提供有关反应的更多信息。它们可用于筛选化合物的活性以及确定IC 50值。

DNA的裂解是拓扑异构酶反应机制的重要组成部分,裂解复合物的稳定化是许多抑制剂(例如喹诺酮和依托泊苷)活性的关键。切割测定可用于确定化合物在稳定切割复合物中的活性。

DNA展开分析可用于确定化合物是否为嵌入剂。


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