P. aeruginosa Gyrase

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P. aeruginosa Gyrase

Pseudomonas aeruginosa gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A₂B₂ complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

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Select a document...P.aeruginosa Gyrase Supercoiling Assay ProtocolP.aeruginosa Gyrase Data SheetP.aeruginosa Gyrase MSDSP.aeruginosa Gyrase lot#030317 Certificate of AnalysisP.aeruginosa gyrase enzyme lot 241017 Certifcate of AnalysisP.aeruginosa gyrase supercoiling kit lot 241017 Certificate of AnalysisP.aeruginosa gyrase Certificate of Analysis lot #280318View

Cat No.	Product	Price	Quantity
PAG1001	P. aeruginosa Gyrase100 U	£110
PAG5002	P. aeruginosa Gyrase500 U	£405

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P. aeruginosa Gyrase Supercoiling Assay Kits

Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers. 

Technical Documents

Select a document to view

Select a document...P.aeruginosa Gyrase Supercoiling Assay ProtocolP.aeruginosa Gyrase Supercoiling Kit Data SheetP.aeruginosa Gyrase Supercoiling Kit MSDSP.aeruginosa Gyrase Supercoiling Kits lot#030317 Certificate of AnalysisP.aeruginosa gyrase supercoiling kit lot 241017 Certificate of AnalysisView

Cat No.	Product	Price	Quantity
PAS001	P.aeruginosa Gyrase Supercoiling Assay Kit 1100 U of P. aeruginosa gyrase and 50 µg of relaxed DNA	£191
PAS002	P.aeruginosa Gyrase Supercoiling Kit 2500 U of P. aeruginosa gyrase and 50 µg of relaxed DNA	£725

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Inspiralis

Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。

We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.

Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.

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