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inspiralis/S. aureus Topoisomerase IV/500 U of S. aureus topo IV and 100µg of kDNA/SAD4002

inspiralis/S. aureus Topoisomerase IV/500 U of S. aureus topo IV and 100µg of kDNA/SAD4002
  • inspiralis/S. aureus Topoisomerase IV/500 U of S. aureus topo IV and 100µg of kDNA/SAD4002
商品介绍

S. aureus Topoisomerase IV

Click on the product headings below to view details and order online.

S. aureus Topoisomerase IV

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Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
SAT4001S. aureus Topo IV100 U£135
SAT4002S. aureus Topo IV500 U£499
Custom Quote

S. aureus Topoisomerase IV Relaxation Assay Kits

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These contain S. aureus topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 

Technical Documents

Cat No.ProductPriceQuantity
SAR4001S.aureus Topo IV RelaxationAssay Kit 1100 U of S. aureus Topo IV and 50 µg of Supercoiled DNA£220
SAR4002S.aureus Topo IV RelaxationAssay Kit 2500 U of S. aureus Topo IV and 50 µg of Supercoiled DNA£816
Custom Quote

S. aureus Topoisomerase IV Decatenation Assay Kits

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These contain S. aureus topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. 

Technical Documents

Cat No.ProductPriceQuantity
SAD4001S.aureus Topo IV Decatenation Assay Kit 1100 U of S. aureus topo IV and 20 µg of kDNA£220
SAD4002S.aureus Topo IV Decatenation Assay Kit 2500 U of S. aureus topo IV and 100µg of kDNA£816
Custom Quote

S. aureus Topoisomerase IV Cleavage Assay Kits

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These kits are designed specifically for cleavage reactions. They contain S. aureus topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to linearised pBR322 marker.

Cleavage specific enzyme available separately on request.

Technical Documents

Cat No.ProductPriceQuantity
SATC001S.aureus Topo IV Cleavage Assay Kit 1100 U of S. aureus topo IV and 50 ug of supercoiled DNA£399
Custom Quote

S. aureus Topoisomerase IV Assay Kit for Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed S. aureus topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.

Technical Documents

Cat No.ProductPriceQuantity
SATE001S.aureus Topo IV Cell Extract Assay Kit 1For 100 assays£136
SATE002S.aureus Topo IV Cell Extract Assay Kit 2For 500 assays£408
Custom Quote

S. aureus Topoisomerase IV ATPase kit

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These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of topoisomerase IV by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling.

These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.

Technical Documents

Cat No.ProductPriceQuantity
ATPSAT001Kit for 100 ATPase assaysFor determination of DNA-dependent ATPase activity; contains E. coli topoIV, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.£1,402
Custom Quote

High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV

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The kit is supplied with sufficient S. aureus topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
SATRIV01HTS Assay Kit S. aureus Topo IV 1 - 5 KitsFor 100 assays per plate£440
SATRIV02HTS Assay Kit S. aureus Topo IV 6+ KitsFor 100 assays per plate£346
Custom Quote
品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

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inspiralis大肠杆菌促旋酶


DNA促旋酶是一种细菌II型拓扑异构酶,可将负超螺旋引入DNA。它是喹诺酮和香豆素药物的靶标,可用于筛选潜在的抗菌化合物。它由过量生产的菌株JMtacA和JMtacB 1制备, 并以A 2 B 2  复合物形式提供。

储存在-80°C。

所有酶均随附5X浓缩测定缓冲液和稀释缓冲液,它们也可以单独购买。1 U的促旋酶将在37°C的30分钟内超螺旋0.5 µg松弛的pBR322 DNA。


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