S. aureus Topoisomerase IV
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S. aureus Topoisomerase IV
Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
See technical documents below for more detailed information and lot specific activities.
Technical Documents
S. aureus Topoisomerase IV Relaxation Assay Kits
These contain S. aureus topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
Technical Documents
S. aureus Topoisomerase IV Decatenation Assay Kits
These contain S. aureus topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
Technical Documents
S. aureus Topoisomerase IV Cleavage Assay Kits
These kits are designed specifically for cleavage reactions. They contain S. aureus topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to linearised pBR322 marker.
Cleavage specific enzyme available separately on request.
Technical Documents
S. aureus Topoisomerase IV Assay Kit for Cell Extracts
These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed S. aureus topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.
Technical Documents
S. aureus Topoisomerase IV ATPase kit
These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of topoisomerase IV by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling.
These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.
Technical Documents
High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV
The kit is supplied with sufficient S. aureus topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.
More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".
Kit issued with limited licence for individual use only.
Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)
Technical Documents
Inspiralis
Inspiralis在提供高质量拓扑异构酶，连接酶和解旋酶方面享誉全球，包括检测试剂盒，底物和筛选服务。
We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.
Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.
产品列表：
No. | 品牌 | 货号 | 名称 | 规格 |
1 | Inspiralis | 7F001 | 7F11 Monoclonal | 0.1ml |
2 | Inspiralis | PA001 | Anti-Gyr A Polyclonal | 0.1 ml |
3 | Inspiralis | HT001 | Anti-human topo II polyclonal - 0.1mL | 0.1 ml |
4 | Inspiralis | G1001 | E.coli gyrase | 100 U |
5 | Inspiralis | T4001 | E. coli Topo IV | 100 U |
6 | Inspiralis | SAG4001 | S. aureus gyrase | 100 U |
7 | Inspiralis | SAT4001 | S. aureus Topo IV | 100 U |
8 | Inspiralis | MTG001 | M. tuberculosis Gyrase (HIS) | 100 U |
9 | Inspiralis | MTT01 | M.tuberculosis Topo I | 100 U |
10 | Inspiralis | PAG1001 | P. aeruginosa Gyrase | 100 U |
11 | Inspiralis | PAT4001 | P. aeruginosa Topo IV | 100 U |
12 | Inspiralis | SPG1001 | S. pneumoniae Gyrase | 100 U |
13 | Inspiralis | SPT4001 | S. pneumoniae Topo IV | 100 U |
14 | Inspiralis | ABG1001 | A. baumannii Gyrase | 100 U |
15 | Inspiralis | ABT4001 | A. baumannii Topo IV | 100 U |
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