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℡ 4000-520-616
℡ 4000-520-616
inspiralis/S. aureus Topoisomerase IV/500 U/SAT4002
产品编号:SAT4002
市  场 价:¥9980.00
场      地:美国(厂家直采)
产品分类: 仪器类>计数测量>酶标仪>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$499.00
品      牌: inspiralis
公      司:inspiralis
公司分类:
inspiralis/S. aureus Topoisomerase IV/500 U/SAT4002
商品介绍

S. aureus Topoisomerase IV

Click on the product headings below to view details and order online.

S. aureus Topoisomerase IV

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Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
SAT4001S. aureus Topo IV100 U£135
SAT4002S. aureus Topo IV500 U£499
Custom Quote

S. aureus Topoisomerase IV Relaxation Assay Kits

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These contain S. aureus topo IV and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo IV will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 

Technical Documents

Cat No.ProductPriceQuantity
SAR4001S.aureus Topo IV RelaxationAssay Kit 1100 U of S. aureus Topo IV and 50 µg of Supercoiled DNA£220
SAR4002S.aureus Topo IV RelaxationAssay Kit 2500 U of S. aureus Topo IV and 50 µg of Supercoiled DNA£816
Custom Quote

S. aureus Topoisomerase IV Decatenation Assay Kits

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These contain S. aureus topo IV and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. 

Technical Documents

Cat No.ProductPriceQuantity
SAD4001S.aureus Topo IV Decatenation Assay Kit 1100 U of S. aureus topo IV and 20 µg of kDNA£220
SAD4002S.aureus Topo IV Decatenation Assay Kit 2500 U of S. aureus topo IV and 100µg of kDNA£816
Custom Quote

S. aureus Topoisomerase IV Cleavage Assay Kits

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These kits are designed specifically for cleavage reactions. They contain S. aureus topo IV enzyme, supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to linearised pBR322 marker.

Cleavage specific enzyme available separately on request.

Technical Documents

Cat No.ProductPriceQuantity
SATC001S.aureus Topo IV Cleavage Assay Kit 1100 U of S. aureus topo IV and 50 ug of supercoiled DNA£399
Custom Quote

S. aureus Topoisomerase IV Assay Kit for Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing over-expressed S. aureus topo IV and contain supercoiled DNA substrate, Assay buffer, Dilution buffer, control relaxed DNA and stop buffer/loading dye.

Technical Documents

Cat No.ProductPriceQuantity
SATE001S.aureus Topo IV Cell Extract Assay Kit 1For 100 assays£136
SATE002S.aureus Topo IV Cell Extract Assay Kit 2For 500 assays£408
Custom Quote

S. aureus Topoisomerase IV ATPase kit

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These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of topoisomerase IV by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling.

These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.

Technical Documents

Cat No.ProductPriceQuantity
ATPSAT001Kit for 100 ATPase assaysFor determination of DNA-dependent ATPase activity; contains E. coli topoIV, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.£1,402
Custom Quote

High / Medium-Throughput Assay Kit - S. aureus Topoisomerase IV

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The kit is supplied with sufficient S. aureus topo IV enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
SATRIV01HTS Assay Kit S. aureus Topo IV 1 - 5 KitsFor 100 assays per plate£440
SATRIV02HTS Assay Kit S. aureus Topo IV 6+ KitsFor 100 assays per plate£346
Custom Quote
品牌介绍

Inspiralis


Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。


We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.


Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.


产品列表:



No.

品牌

货号

名称

规格

1

Inspiralis

7F001

7F11 Monoclonal

0.1ml

2

Inspiralis

PA001

Anti-Gyr A Polyclonal

0.1 ml

3

Inspiralis

HT001

Anti-human topo II polyclonal - 0.1mL

0.1 ml

4

Inspiralis

G1001

E.coli gyrase

100 U

5

Inspiralis

T4001

E. coli Topo IV

100 U

6

Inspiralis

SAG4001

S. aureus gyrase

100 U

7

Inspiralis

SAT4001

S. aureus Topo IV

100 U

8

Inspiralis

MTG001

M. tuberculosis Gyrase (HIS)

100 U

9

Inspiralis

MTT01

M.tuberculosis Topo I

100 U

10

Inspiralis

PAG1001

P. aeruginosa Gyrase

100 U

11

Inspiralis

PAT4001

P. aeruginosa Topo IV

100 U

12

Inspiralis

SPG1001

S. pneumoniae Gyrase

100 U

13

Inspiralis

SPT4001

S. pneumoniae Topo IV

100 U

14

Inspiralis

ABG1001

A. baumannii Gyrase

100 U

15

Inspiralis

ABT4001

A. baumannii Topo IV

100 U



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