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inspiralis/S. aureus Gyrase/For 500 S. aureus gyrase assays/SAGE002

inspiralis/S. aureus Gyrase/For 500 S. aureus gyrase assays/SAGE002
  • inspiralis/S. aureus Gyrase/For 500 S. aureus gyrase assays/SAGE002
商品介绍

S. aureus Gyrase

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S. aureus Gyrase

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Staphylococcus aureus gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
SAG4001S. aureus gyrase100 U£110
SAG4002S. aureus gyrase500 U£405
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S. aureus Gyrase Supercoiling Assay Kits

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Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers.

Technical Documents

Cat No.ProductPriceQuantity
SAS4001S.aureus Gyrase Supercoiling Assay Kit 1100 U of S. aureus gyrase and 50 µg of relaxed DNA£191
SAS4002S.aureus Gyrase Supercoiling Assay Kit 2500 U of S. aureus gyrase and 250 µg of relaxed DNA£704
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S. aureus Gyrase Cleavage Assay Kits

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Some drugs interrupt the DNA breakage-reunion step of the gyrase reaction. This leads to cell death and it is the mechanism behind the action of the quinolones such as nalidixic acid and ciprofloxacin. Cleavage assays are particularly useful in determining if a potential drug acts by this mechanism.

These kits are designed specifically for cleavage reactions. They contain the supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to the S. aureus gyrase and linearised pBR322 marker.

Cleavage specific enzyme available separately on request.

Technical Documents

Cat No.ProductPriceQuantity
SAGC001S.aureus Gyrase Cleavage Assay Kit 1100 U of S. aureus gyrase and 50 ug of supercoiled DNA£376
SAGC002S.aureus Gyrase Cleavage Assay Kit 2500 U of S. aureus gyrase and 50 ug of supercoiled DNA£1,065
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S. aureus Gyrase Assay Kits for Cell Extracts

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These kits are designed for assaying cell extracts containing gyrase and partially purified fractions and contain relaxed DNA substrate, Assay buffer, Dilution buffer and control supercoiled DNA. 

Technical Documents

Cat No.ProductPriceQuantity
SAGE001S.aureus Gyrase Cell Extract Assay Kit 1For 100 S. aureus gyrase assays£136
SAGE002S.aureus Gyrase Cell Extract Assay Kit 2For 500 S. aureus gyrase assays£428
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S. aureus Gyrase ATPase Kits

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The coumarin drugs inhibit the action of gyrase by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling. These kits can be used to test the effects of potential ATPase inhibitors.

These kits are advantageous because the assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. 

Technical Documents

Cat No.ProductPriceQuantity
ATPSAG001Kit for 100 ATPase assaysFor determination of DNA-dependent ATPase activity; contains S. aureus gyrase, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.£1,546
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High / Medium-Throughput Assay Kit - S. aureus Gyrase

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The kit is supplied with sufficient S. aureus gyrase enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
SATRG01HTS Assay Kit S. aureus Gyrase 1 - 5 KitsFor 100 assays per plate£410
SATRG02HTS Assay Kit S. aureus Gyrase 6+ KitsFor 100 assays per plate£340
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品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

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inspiralis高/中通量拓扑异构酶测定

John Innes中心的A. Maxwell教授和Inspiralis员工在实验室中开发了一种基于高通量微量滴定板的测定法。基于这样的事实,即负超螺旋质粒比松弛质粒更容易形成分子间三链体DNA。该测定法克服了基于凝胶的测定法的一些问题,这些问题很费时,因此固有地是低通量的。Inspiralis能够提供此测定方法,作为筛选化合物对拓扑异构酶(包括促旋酶,topo IV和人topos I和II)的抑制活性的手段。该测定法适用于改变DNA超螺旋的其他酶,例如限制酶。该测定与常规基于凝胶的测定比较的一些结果如下所示。

该测定法对化合物的筛选特别有用,也可用于测定IC 50  值。在以下给出的实施例中,比较了基于板和凝胶的测定法对陀螺IV和人topo I和II对陀螺酶抑制超螺旋和松弛的IC 50的确定 在两种测定中均获得了相似的抑制谱和IC 50值。


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