请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
inspiralis/E. coli Gyrase/For 100 E. coli gyrase assays/GCE001
产品编号:GCE001
市  场 价:¥2720.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$136.00
品      牌: inspiralis
公      司:inspiralis
公司分类:
inspiralis/E. coli Gyrase/For 100 E. coli gyrase assays/GCE001
商品介绍

E. coli Gyrase

Click on the product headings below to view details and order online.

E. coli Gyrase

>

DNA gyrase is a bacterial type II topisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared from the over producing strains JMtacA and JMtacB1 and is supplied as an A2B2 complex.

Store at -80°C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37°C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
G1001E.coli gyrase100 U£110
G5002E.coli gyrase500 U£405
G1003E.coli gyrase1000 U£784
G2004E.coli gyrase2000 U£1,505
Custom Quote

E. coli Gyrase Supercoiling Assay Kits

>

Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers.

Technical Documents

Cat No.ProductPriceQuantity
K0001E.coli Gyrase Supercoiling Kit 1100 U of E. coli gyrase and 50 µg of relaxed DNA£191
K0002E.coli Gyrase Supercoiling Kit 2500 U of E. Coli gyrase and 250 µg of relaxed DNA£725
K003E.coli Gyrase Supercoiling Kit 31000 U of E. coli gyrase and 500 µg of relaxed DNA£1,447
K004E.coli Gyrase Supercoiling Kit 42000 U of E. coli gyrase and 1 mg of relaxed DNA£2,737
Custom Quote

E. coli Gyrase Cleavage Assay Kits

>

Some drugs interrupt the DNA breakage-reunion step of the gyrase reaction. This leads to cell death and it is the mechanism behind the action of the quinolones such as nalidixic acid and ciprofloxacin. Cleavage assays are particularly useful in determining if a potential drug acts by this mechanism.

These kits are designed specifically for cleavage reactions. They contain the supercoiled pBR322 DNA substrate and the Assay and Dilution buffers required for DNA cleavage reactions in addition to the enzyme and linearised pBR322 marker.

Cleavage specific enzyme available separately on request.

Technical Documents

Cat No.ProductPriceQuantity
GCK001E.coli Gyrase Cleavage Kit 1100 U of E. coli gyrase and 50 µg of supercoiled DNA£376
GCK002E.coli Gyrase Cleavage Kit 2500 U of E. coli gyrase and 250 µg of supercoiled DNA£1,065
GCK003E.coli Gyrase CleavageKit 31000 U of E. coli gyrase and 500 µg of supercoiled DNA£1,470
GCK004E.coli Gyrase CleavageKit 42000 U of E. coli gyrase and 1 mg of supercoiled DNA£2,939
Custom Quote

E. coli Gyrase Assay Kits for Cell Extracts

>

These kits are designed for assaying cell extracts containing E. coli gyrase and partially purified fractions and contain relaxed DNA substrate, Assay buffer, Dilution buffer and control supercoiled DNA. 

Technical Documents

Cat No.ProductPriceQuantity
GCE001E.coli Cell Extract Assay Kit 1For 100 E. coli gyrase assays£136
GCE002E.coli Cell Extract Assay Kit 2For 500 E. coli gyrase assays£402
GCE003E.coli Cell Extract Assay Kit 3For 1000 E. coli gyrase assays£799
GCE004E.coli Cell Extract Assay Kit 4For 2000 E. coli gyrase assays£1,527
Custom Quote

E. coli Gyrase ATPase Kits

>

These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of DNA gyrase by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling. 

These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.

Technical Documents

Cat No.ProductPriceQuantity
ATPG001Kit for 100 ATPase assaysFor determination of DNA-dependent ATPase activity; contains E. coli gyrase, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.£1,546
ATPG002Kit for 100 ATPase assaysFor determination of DNA-independent ATPase activity; contains E. coli GyrB43 domain, buffers, PK-LDH, PEP, NADH and ATP.£1,546
Custom Quote

High / Medium-Throughput Assay Kit - E. coli Gyrase

>

The kit is supplied with sufficient E. coli gyrase enzyme, plasmid DNA substrate, buffers and other assay components for 100 assays. The enzyme is supplied at a concentration of 5 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use. 

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.ProductPriceQuantity
TRG01HTS Assay Kit - E. coli Gyrase 1 - 5 KitsFor 100 assays per plate£399
TRG02HTS Assay Kit - E. coli Gyrase 6+ KitsFor 100 assays per plate£340
Custom Quote

References

  1. Hallett, P., Grimshaw, A.J., Wigley, D.B. and Maxwell, A. (1990) Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins. Gene 93: 139-142
  2. Maxwell, A., Burton, N.P. and O"Hagan, N. (2006) High-throughput assays for DNA gyrase and other topoisomerases. Nucleic Acid Res. 34 (15), e104
品牌介绍

Inspiralis


Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。


We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.


Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.


产品列表:



No.

品牌

货号

名称

规格

1

Inspiralis

7F001

7F11 Monoclonal

0.1ml

2

Inspiralis

PA001

Anti-Gyr A Polyclonal

0.1 ml

3

Inspiralis

HT001

Anti-human topo II polyclonal - 0.1mL

0.1 ml

4

Inspiralis

G1001

E.coli gyrase

100 U

5

Inspiralis

T4001

E. coli Topo IV

100 U

6

Inspiralis

SAG4001

S. aureus gyrase

100 U

7

Inspiralis

SAT4001

S. aureus Topo IV

100 U

8

Inspiralis

MTG001

M. tuberculosis Gyrase (HIS)

100 U

9

Inspiralis

MTT01

M.tuberculosis Topo I

100 U

10

Inspiralis

PAG1001

P. aeruginosa Gyrase

100 U

11

Inspiralis

PAT4001

P. aeruginosa Topo IV

100 U

12

Inspiralis

SPG1001

S. pneumoniae Gyrase

100 U

13

Inspiralis

SPT4001

S. pneumoniae Topo IV

100 U

14

Inspiralis

ABG1001

A. baumannii Gyrase

100 U

15

Inspiralis

ABT4001

A. baumannii Topo IV

100 U



自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔