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inspiralis/Specific Gyrase Protein Domains/0.5 mg/SUBB005

inspiralis/Specific Gyrase Protein Domains/0.5 mg/SUBB005
  • inspiralis/Specific Gyrase Protein Domains/0.5 mg/SUBB005
商品介绍

Specific Gyrase Protein Domains

All our gyrase subunits are supplied at a concentration of 1 mg/ml unless otherwise specified and are purified to > 95% as assessed by SDS-PAGE. We have an extensive collection of mutants not listed here so please contact us for a full list. Different quantities and concentrations can be supplied on request.

Click on the product headings below to view details and order online.

Gyrase Subunits

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For studies involving mutant drug-resistant E. coli gyrase subunits the wild-type subunits are available separately in the following amounts. The subunits are supplied at a concentration of 1 mg/ml, purified to > 95% as assessed by SDS-PAGE. Other quantities and concentrations are available as are the individual subunits from S. aureus and M. tuberculosis gyrases; please enquire for further information.

gyrase subunit 1

Technical Documents

Cat No.ProductPriceQuantity
SUBA001E. coli Gyrase A0.1 mgPOA
SUBA005E. coli Gyrase A0.5 mgPOA
SUBA100E. coli Gyrase A1.0 mgPOA
SUBB001E. coli Gyrase B0.1 mgPOA
SUBB005E. coli Gyrase B0.5 mgPOA
SUBB100E. coli Gyrase B1.0 mgPOA
Custom Quote

Purified Gyrase Domains

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For structural studies the following E. coli gyrase domains are available: GyrB43, GyrB24 and GyrA59. Domains supplied at a concentration of 1 mg/ml purified to > 95% as assessed by SDS-PAGE. (see gel below for typical levels of purity)

Larger quantities/different concentrations are available, please enquire.

gyrase subunit 2

Technical Documents

Cat No.ProductPriceQuantity
A59001Gyrase A590.1 mgPOA
A59005Gyrase A590.5 mgPOA
A59100Gyrase A591.0 mgPOA
B43001Gyrase B430.1 mgPOA
B43005Gyrase B430.5 mgPOA
B43100Gyrase B431.0 mgPOA
B24001Gyrase B240.1 mgPOA
B24005Gyrase B240.5 mgPOA
B24100Gyrase B241.0 mgPOA
Custom Quote

Drug-Resistant Topoisomerases

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We offer various quinolone- and coumarin-resistant topoisomerase mutants includingE.coliS.aureus and other organisms. The proteins can be supplied as separate subunits or as reconstituted enzyme. Typically the quinolone-resistant mutants confer 50-100 X more resistance to fluoroquinolones than the wild-type. We have more than 25 mutants in different topoisomerases and can make other mutants as requested. 

Technical Documents

Cat No.ProductPriceQuantity
AS83W001E.coli GyrASer83TrpPlease enquirePOA
AS83L001E.coli GyrASer83LeuPlease enquirePOA
BD426N001E.coli GyrBAsp426AsnPlease enquirePOA
SAAS84L01S.aureus GyrASer84LeuPlease enquirePOA
Custom Quote
品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



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inspiralis拓扑异构酶抑制剂的作用机理

拓扑异构酶抑制剂可能以多种方式起作用或在蛋白质的不同位点结合。例如,它们可以抑制酶的ATPase活性,充当裂解复合物稳定剂,嵌入DNA中或阻止DNA结合。我们可以进行不同的测定,这将有助于阐明抑制剂的作用方式。


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