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inspiralis/Human Topoisomerase I/500 U of human topo I and 250 µg of supercoiled DNA/HTR102

inspiralis/Human Topoisomerase I/500 U of human topo I and 250 µg of supercoiled DNA/HTR102
  • inspiralis/Human Topoisomerase I/500 U of human topo I and 250 µg of supercoiled DNA/HTR102
商品介绍

Human Topoisomerase I

Click on the product headings below to view details and order online.

Human Topoisomerase I

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Human Topoisomerase I is prepared by overexpressing the enzyme in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods adapted from Stewart et al., 1996. The enzyme is supplied μl in Dilution Buffer.

Store at -80°C. It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.ProductPriceQuantity
HT101Human Topo I100 U£75
HT105Human Topo I500 U£230
HT110Human Topo I1000 U£385
HT120Human Topo I2000 U£640
Custom Quote

Human Topoisomerase I Relaxation Assay Kits

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These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 

Technical Documents

Cat No.ProductPriceQuantity
HTR101Human Topo I Relaxation Assay Kit 1100 U of human topo I and 50 µg of supercoiled DNA£195
HTR102Human Topo I Relaxation AssayKit 2500 U of human topo I and 250 µg of supercoiled DNA£615
HTR103Human Topo I Relaxation AssayKit 31000 U of human topo I and 500 µg of supercoiled DNA£1,150
HTR104Human Topo I Relaxation AssayKit 42000 U of human topo I and 1 mg of supercoiled DNA£1,800
Custom Quote

Human Topoisomerase I Assay Kits for Cell Extracts

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These kits are designed for assaying cell extracts and partially purified fractions containing human topo I. They contain supercoiled DNA substrate, Assay buffer, Dilution buffer and relaxed DNA marker.

The kit components are based on the standard human topo I assay whch contains 500 ng substrate DNA per assay.

Technical Documents

Cat No.ProductPriceQuantity
TRAK1Human Topo I Cell Extract Assay Kit 1Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 100 assays£162
TRAK2Human Topo I Cell Extract AssayKit 2Contains sufficient assay buffers, supercoiled and relaxed marker DNA for 250 assays£409
Custom Quote

High / Medium-Throughput Assay Kit - Human Topoisomerase I

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The kit is supplied with sufficient human topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230).

Technical Documents

Cat No.ProductPriceQuantity
TRT101HTS Assay Kit - Human Topo I 1-5 kitsfor 100 assays per plate£430
TRT102HTS Assay Kit - Human Topo I 6+ Kitsfor 100 assays per plate£346
Custom Quote
品牌介绍

自1995年以来,我们一直为制药行业和学术界提供拓扑异构酶产品和服务,以协助抗感染和抗癌市场的研究。Inspiralis以前是John Innes Enterprises的公司,它是根据拓扑异构酶研究领域的全球领先者之一Tony Maxwell教授的研究建立的。

我们位于约翰·因内斯中心和东英吉利大学诺里奇研究园的学术研究中心附近的实验室。我们与麦克斯韦教授的学术研究小组保持着密切的联系,帮助我们保持在拓扑异构酶领域的最前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持,以确保研究人员能够使用我们的产品获得最佳结果。生产我们的酶和底物的科学家也提供技术支持。

我们的目标是为制药公司和其他参与药物开发的公司提供开发和筛选新型抗感染和抗癌化合物的必要工具。为了实现这一目标,我们将继续扩大拓扑异构酶产品和筛选服务的范围。



蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

【订购】蚂蚁淘生物科技  联系方式:4000-520-616

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inspiralis基于凝胶的分析

我们提供一系列基于凝胶的测定法,并具有多年与制药公司和学术界的客户合作的经验。

  • DNA旋转酶超螺旋

  • DNA拓扑异构酶I松弛

  • DNA拓扑异构酶II松弛

  • DNA拓扑异构酶II变性

  • DNA拓扑异构酶IV松弛

  • DNA拓扑异构酶IV脱落

这些都使用琼脂糖凝胶将反应产物与底物分离。在管中进行超螺旋和松弛反应,然后使用琼脂糖凝胶分离产物。分离使得可以看到不同的拓扑异构酶中间体以及超螺旋和松弛质粒。

蜕皮反应可测量从kris中的小环和大环复合物中DNA小环的解链,该复合体已从Crithidia fascicula中分离出来  与松弛反应相比,某些酶在脱脂中具有更高的活性。

与基于微量滴定板的测定相比,基于凝胶的测定的结果的视觉性质可提供有关反应的更多信息。它们可用于筛选化合物的活性以及确定IC 50值。

DNA的裂解是拓扑异构酶反应机制的重要组成部分,裂解复合物的稳定化是许多抑制剂(例如喹诺酮和依托泊苷)活性的关键。切割测定可用于确定化合物在稳定切割复合物中的活性。

DNA展开分析可用于确定化合物是否为嵌入剂。


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