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Mycobacterium tuberculosis gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the subunits with C-terminal polyhistidine tags in E. coli and then purified by affinity chromatography.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.
See technical documents below for more detailed information and lot specific activities.
Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain M. tuberculosis gyrase, relaxed DNA substrate and the Assay and Dilution buffers.
These contain the M. tuberculosis gyrase and the kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of M. tuberculosis gyrase will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
These kits are designed for assaying cell extracts containing M. tuberculosis gyrase and partially purified fractions and contain relaxed DNA substrate, Assay buffer, Dilution buffer and control supercoiled DNA.
Inspiralis
Inspiralis在提供高质量拓扑异构酶,连接酶和解旋酶方面享誉全球,包括检测试剂盒,底物和筛选服务。
We are located in laboratories adjacent to the academic research centres of the John Innes Centre and the University of East Anglia on the Norwich Research Park. We maintain close links with the academic research group of Professor Maxwell, helping us to remain at the forefront of the topoisomerase field. The experience of our staff and the connections with the academic group allow us to provide a high level of support to ensure that researchers are able to get the best results using our products. The same scientists who produce our enzymes and substrates also provide the technical support.
Our aim is to provide pharmaceutical companies, and others involved in drug development, with the necessary tools to develop and screen novel anti-infective and anti-cancer compounds. In order to achieve this we will continue to expand the range of topoisomerase products and screening services we offer.
产品列表:
No. | 品牌 | 货号 | 名称 | 规格 |
1 | Inspiralis | 7F001 | 7F11 Monoclonal | 0.1ml |
2 | Inspiralis | PA001 | Anti-Gyr A Polyclonal | 0.1 ml |
3 | Inspiralis | HT001 | Anti-human topo II polyclonal - 0.1mL | 0.1 ml |
4 | Inspiralis | G1001 | E.coli gyrase | 100 U |
5 | Inspiralis | T4001 | E. coli Topo IV | 100 U |
6 | Inspiralis | SAG4001 | S. aureus gyrase | 100 U |
7 | Inspiralis | SAT4001 | S. aureus Topo IV | 100 U |
8 | Inspiralis | MTG001 | M. tuberculosis Gyrase (HIS) | 100 U |
9 | Inspiralis | MTT01 | M.tuberculosis Topo I | 100 U |
10 | Inspiralis | PAG1001 | P. aeruginosa Gyrase | 100 U |
11 | Inspiralis | PAT4001 | P. aeruginosa Topo IV | 100 U |
12 | Inspiralis | SPG1001 | S. pneumoniae Gyrase | 100 U |
13 | Inspiralis | SPT4001 | S. pneumoniae Topo IV | 100 U |
14 | Inspiralis | ABG1001 | A. baumannii Gyrase | 100 U |
15 | Inspiralis | ABT4001 | A. baumannii Topo IV | 100 U |